| 1 | Anglo American Services (UK) Ltd. London Turnover £267.0M | £12.4bn 2022 accounts | £267.0M |
| 2 | Horse Powertrain Limited London Turnover £7.4M | £7.6bn 2024 accounts | £7.4M |
| 3 | Canary Wharf Group PLC London | £2.2bn 2024 accounts | — |
| 4 | Leonardo Hotel Management (UK) Limited Turnover £345.5M | £1.5bn 2024 accounts | £345.5M |
| 5 | Inspiring Vet Care Limited Bristol Turnover £667.4M | £863.7M 2020 accounts | £667.4M |
| 6 | National Oilwell Varco UK Limited Stonehouse Turnover £374.1M | £837.2M | £374.1M |
| 7 | Rampion Offshore Wind Limited Swindon | £740.1M | — |
| 8 | Haydock Finance Limited Blackburn | £695.7M | — |
| 9 | Steris Limited Basingstoke | £628.0M | — |
| 10 | Willis Limited London Turnover £1.1bn | £615.0M | £1.1bn |
| 11 | Sopra Steria Limited London Turnover £436.4M | £497.4M | £436.4M |
| 12 | Sodexo Limited London Turnover £1.4bn | £487.3M | £1.4bn |
| 13 | Thomson Reuters (Professional) UK Limited London Turnover £590.2M | £473.7M 2023 accounts | £590.2M |
| 14 | Croda Europe Limited East Yorkshire Turnover £432.4M | £458.5M | £432.4M |
| 15 | Quadrature Capital Limited London Turnover £1.2bn | £398.8M 2025 accounts | £1.2bn |
| 16 | Shotton Mill Limited Deeside Turnover £38.5M | £396.2M 2023 accounts | £38.5M |
| 17 | Ogilvie Fleet Limited Stirling | £370.2M | — |
| 18 | Thameswey Housing Limited London Turnover £13.9M | £355.4M 2024 accounts | £13.9M |
| 19 | Rapiscan Systems Limited London | £344.4M | — |
| 20 | N-Able International Ltd London | £322.7M 2024 accounts | — |
| 21 | N-Able Solutions Ltd London | £311.8M | — |
| 22 | Aesop UK Limited London | £302.7M | — |
| 23 | Bakkafrost Scotland Limited Edinburgh | £297.4M 2023 accounts | — |
| 24 | Amey Holdings Limited London | £293.9M | — |
| 25 | Trailfinders Limited | £292.0M 2022 accounts | — |
| 26 | Orbis Education and Care Limited Cardiff Turnover £33.0M | £264.1M | £33.0M |
| 27 | Bio Capital Ltd Buntingford | £256.2M 2024 accounts | — |
| 28 | Azets Holdings Limited London | £239.6M | — |
| 29 | Warren James (Jewellers) Limited | £239.5M | — |
| 30 | Shanly Homes Limited Beaconsfield Turnover £57.6M | £236.5M 2023 accounts | £57.6M |
| 31 | Code Students Limited Leicester Turnover £19.4M | £228.6M 2024 accounts | £19.4M |
| 32 | O&H Properties Limited London | £221.7M 2025 accounts | — |
| 33 | Admiral Taverns Limited London Turnover £67.6M | £220.9M | £67.6M |
| 34 | Gladman Developments Limited Congleton Turnover £29.9M | £207.4M 2021 accounts | £29.9M |
| 35 | John Fowler Holidays Limited Barnstaple Turnover £37.1M | £205.4M | £37.1M |
| 36 | Energizer Trading Limited High Wycombe Turnover £339.2M | £204.6M | £339.2M |
| 37 | Causeway Technologies Limited Gerrards Cross | £203.2M | — |
| 38 | Enra Specialist Finance Limited Watford Turnover £904.9k | £203.1M | £904.9k |
| 39 | Reuters News & Media Limited London Turnover £34.0M | £202.9M 2024 accounts | £34.0M |
| 40 | Sonatrach Gas Marketing UK Limited London | £199.9M 2024 accounts | — |
| 41 | Weston Importers Ltd. Edinburgh | £199.6M | — |
| 42 | Cavendish Education Limited London Turnover £20.2M | £187.5M | £20.2M |
| 43 | James Hall and Company Limited Preston | £186.3M | — |
| 44 | Amey Defence Services Limited London Turnover £138.3M | £182.8M | £138.3M |
| 45 | Amey Limited London | £175.3M | — |
| 46 | Wernick Hire Limited Wickford | £174.8M 2024 accounts | — |
| 47 | Marston Properties Limited London Turnover £7.9M | £171.5M 2023 accounts | £7.9M |
| 48 | LWC Drinks Limited Manchester | £166.8M | — |
| 49 | The Energy Saving Trust Limited London Turnover £83.5M | £163.9M 2023 accounts | £83.5M |
| 50 | Shop TJC Limited Staines-Upon-Thames Turnover £78.9M | £161.7M 2024 accounts | £78.9M |
| 51 | WJ Group Infrastructure Limited Newcastle | £159.5M | — |
| 52 | Penguin Books Limited London Turnover £247.2M | £157.7M 2024 accounts | £247.2M |
| 53 | Saria Limited Doncaster Turnover £3.4M | £156.4M 2024 accounts | £3.4M |
| 54 | Charles Wilson Engineers Limited St Albans | £147.7M | — |
| 55 | Colleycare Limited Hemel Hempstead Turnover £65.7M | £147.3M | £65.7M |
| 56 | Pickerings Hire Limited Swadlincote Turnover £70.6M | £143.6M 2024 accounts | £70.6M |
| 57 | Amey Owr Limited London Turnover £56.2M | £141.5M | £56.2M |
| 58 | Nottingham Forest Football Club Limited West Bridgford | £140.1M | — |
| 59 | Practice Plus Group Holdings Limited Reading Turnover £0 | £139.9M 2019 accounts | £0 |
| 60 | Marcegaglia Stainless Sheffield Ltd Sheffield Turnover £664.0M | £139.8M 2024 accounts | £664.0M |
| 61 | Amey Rail Limited London Turnover £229.9M | £138.4M | £229.9M |
| 62 | Evelyn Partners Services Limited London Turnover £406.3M | £131.3M 2023 accounts | £406.3M |
| 63 | Contino Solutions Limited London | £131.2M 2023 accounts | — |
| 64 | Inpost UK Limited London | £124.4M 2024 accounts | — |
| 65 | Research Instruments Limited Falmouth Turnover £18.7M | £115.9M 2021 accounts | £18.7M |
| 66 | Cyden Homes Limited Grimsby Turnover £58.1M | £115.7M 2024 accounts | £58.1M |
| 67 | Park's of Hamilton (Holdings) Limited Hamilton | £114.8M 2024 accounts | — |
| 68 | FP McCann Limited Magherafelt, | £113.0M | — |
| 69 | Ipswich Town Football Club Company Limited | £112.5M | — |
| 70 | Jack Sealey Limited Colchester Turnover £93.8M | £109.9M | £93.8M |
| 71 | Menzies Aviation Limited London | £106.7M 2023 accounts | — |
| 72 | Kinetic Games Limited Southampton | £106.3M | — |
| 73 | Shared Services Connected Ltd London Turnover £334.1M | £102.7M | £334.1M |
| 74 | Rainham Steel Company Limited Romford | £101.4M | — |
| 75 | Magellan Services UK Limited Nottingham Turnover £81.3M | £99.6M 2023 accounts | £81.3M |
| 76 | Gulfstream Aerospace, Ltd London | £99.0M 2024 accounts | — |
| 77 | Ebblo UK Limited Chippenham Turnover £12.0M | £96.8M 2024 accounts | £12.0M |
| 78 | Amey Fleet Services Limited London Turnover £61.4M | £96.8M | £61.4M |
| 79 | M and M Direct Limited Hereford Turnover £306.6M | £96.7M 2021 accounts | £306.6M |
| 80 | Amey Community Limited London Turnover £434.4M | £96.1M | £434.4M |
| 81 | Dhamecha Foods Limited Middlesex | £96.0M 2024 accounts | — |
| 82 | Metro Shipping Limited Birmingham Turnover £169.7M | £92.6M | £169.7M |
| 83 | HFD Datavita Limited Glasgow Turnover £11.9M | £91.9M | £11.9M |
| 84 | U-Pol Limited Wellingborough | £90.6M 2024 accounts | — |
| 85 | Robert Scott & Sons Limited Nr. Oldham, Lancs. | £89.1M | — |
| 86 | Practice Plus Group Hospitals Limited Bristol Turnover £119.6M | £89.0M 2019 accounts | £119.6M |
| 87 | Watford Association Football Club Limited(the) Watford Hertfordshire | £88.1M | — |
| 88 | Anaplan Limited Bristol Turnover £343.5M | £87.9M | £343.5M |
| 89 | Merthyr (South Wales) Limited Newport | £87.1M | — |
| 90 | Gismart Limited London Turnover £61.3M | £86.9M 2020 accounts | £61.3M |
| 91 | Lakeside Energy From Waste Limited Wallingford | £86.7M | — |
| 92 | Oikos Storage Limited Canvey Island Turnover £23.4M | £86.3M | £23.4M |
| 93 | Eland Cables Limited London | £85.9M | — |
| 94 | Harley-Davidson Europe Limited Abingdon Turnover £523.4M | £85.4M 2024 accounts | £523.4M |
| 95 | Rockley Dene Homes Limited Middlesex | £85.2M | — |
| 96 | Magna Specialist Confectioners Limited Telford Turnover £183.7M | £84.8M | £183.7M |
| 97 | Lightspeed Networks Ltd Peterborough | £84.5M 2024 accounts | — |
| 98 | MC Rental Limited Aylesford Turnover £38.5M | £82.5M 2024 accounts | £38.5M |
| 99 | Xtrac Limited Thatcham Turnover £45.9M | £82.1M 2020 accounts | £45.9M |
| 100 | Charles River Laboratories Edinburgh Limited Turnover £127.9M | £80.2M | £127.9M |
| 101 | Morning Foods,limited Crewe Turnover £284.1M | £79.9M | £284.1M |
| 102 | Siva Plastics Limited Woolston | £79.5M | — |
| 103 | Aerotron Limited Crawley | £79.1M 2022 accounts | — |
| 104 | Truespeed Communications Ltd Bath | £79.0M | — |
| 105 | Seras Energy Ltd Widnes Turnover £101.7M | £79.0M 2024 accounts | £101.7M |
| 106 | Hayley Group Limited West Midlands Turnover £232.8M | £78.7M 2023 accounts | £232.8M |
| 107 | Elliott Baxter & Company Limited Farnborough Turnover £178.8M | £78.6M 2024 accounts | £178.8M |
| 108 | Qualis Group Ltd Loughton | £78.0M | — |
| 109 | Clarity Travel Limited Manchester Turnover £32.8M | £77.5M 2023 accounts | £32.8M |
| 110 | Ready Egg Products Limited Enniskillen Turnover £92.0M | £77.4M | £92.0M |
| 111 | Blue Anchor Leisure Limited Skegness Turnover £28.4M | £77.1M 2024 accounts | £28.4M |
| 112 | Rushcliffe Care Limited Loughborough | £75.0M 2024 accounts | — |
| 113 | Arkell's Brewery Limited Swindon Turnover £29.1M | £74.9M 2024 accounts | £29.1M |
| 114 | CMS Distribution Limited London Turnover £312.7M | £74.7M | £312.7M |
| 115 | Tide Construction Limited Marylebone Turnover £177.5M | £74.6M 2022 accounts | £177.5M |
| 116 | Naturalmotion Games Limited London | £74.1M 2023 accounts | — |
| 117 | Anglo American Foundation London | £74.0M 2022 accounts | — |
| 118 | Ralawise Limited Deeside Turnover £168.3M | £74.0M 2024 accounts | £168.3M |
| 119 | The Halcyon Gallery Limited London | £73.8M 2024 accounts | — |
| 120 | Allen Ford (UK) Limited Warwick Turnover £669.3M | £73.6M | £669.3M |
| 121 | Randolph Hill Nursing Homes (Scotland) Limited Edinburgh | £73.2M | — |
| 122 | Cepac Limited London Turnover £116.5M | £73.1M 2024 accounts | £116.5M |
| 123 | Cohort Capital Ltd London Turnover £60.3M | £73.0M 2024 accounts | £60.3M |
| 124 | JJ Food Service Limited Enfield | £72.8M | — |
| 125 | Lake District Hotels Limited Keswick Turnover £29.0M | £72.7M 2025 accounts | £29.0M |
| 126 | Rodericks Dental Partners Limited Northampton Turnover £56.9M | £71.9M 2021 accounts | £56.9M |
| 127 | International Convention Centre Wales Limited Newport | £71.4M | — |
| 128 | Malhotra Care Homes Limited Newcastle Upon Tyne Turnover £21.5M | £70.9M 2021 accounts | £21.5M |
| 129 | Community Health and Eyecare Limited Preston Turnover £94.3M | £70.9M | £94.3M |
| 130 | Wisetech Global (UK) Ltd. London Turnover £23.0M | £70.8M 2024 accounts | £23.0M |
| 131 | Oxford Aviation Services Limited London Turnover £16.5M | £70.3M 2024 accounts | £16.5M |
| 132 | McAleer & Rushe Contracts UK Limited London Turnover £627.7M | £69.9M | £627.7M |
| 133 | Fibre Assets Limited London Turnover £1.4M | £69.4M | £1.4M |
| 134 | Valli Forecourts Limited Wakefield | £68.2M | — |
| 135 | Norfolk Homes Limited London Turnover £53.6M | £68.1M 2025 accounts | £53.6M |
| 136 | Hopkins Estates Limited Wincanton Turnover £17.1M | £68.1M 2024 accounts | £17.1M |
| 137 | Isle of Arran Distillers Limited Lochranza | £68.1M | — |
| 138 | W B Power Services Limited Heanor Turnover £131.4M | £67.9M | £131.4M |
| 139 | Aspire Pharma Limited Godalming | £66.9M 2024 accounts | — |
| 140 | Baker Estates Limited Newton Abbot | £66.8M | — |
| 141 | David Cover and Son Limited Chichester | £65.8M 2024 accounts | — |
| 142 | Optical Express Limited Glasgow | £65.8M 2024 accounts | — |
| 143 | Stanmore Contractors Limited Erith | £65.6M | — |
| 144 | Siderise (Holdings) Limited Maesteg | £65.4M 2024 accounts | — |
| 145 | Karan Retail Ltd Turnover £194.9M | £65.1M | £194.9M |
| 146 | Tetrosyl Limited Rochdale Turnover £90.7M | £64.8M | £90.7M |
| 147 | Gymshark Ltd Solihull Turnover £260.7M | £64.5M 2020 accounts | £260.7M |
| 148 | Okta UK Ltd London | £64.2M 2025 accounts | — |
| 149 | Thameswey Energy Limited London Turnover £7.6M | £64.2M 2024 accounts | £7.6M |
| 150 | Barfoots of Botley Limited Bognor Regis | £64.0M 2024 accounts | — |
| 151 | Direct Table Foods Limited Bury St Edmunds Turnover £143.3M | £63.7M 2024 accounts | £143.3M |
| 152 | Lomas Distribution Limited Buxton | £63.5M | — |
| 153 | Nationwide Finance Limited Olney Turnover £32.6M | £63.1M 2024 accounts | £32.6M |
| 154 | G.H. Dean & Co. Limited Sittingbourne | £63.1M | — |
| 155 | Crew Clothing Co. Limited Kingston Upon Thames Turnover £123.4M | £62.9M 2024 accounts | £123.4M |
| 156 | Heygates Limited Northampton Turnover £250.4M | £62.2M | £250.4M |
| 157 | Nimax Theatres Limited London | £61.9M | — |
| 158 | Thomas Tunnock Limited | £61.8M 2024 accounts | — |
| 159 | George Pragnell Limited Stratford-Upon-Avon | £61.3M | — |
| 160 | Ferdinand Bilstein UK Limited Chesterfield Turnover £121.5M | £60.6M 2024 accounts | £121.5M |
| 161 | Belstaff International Limited London Turnover £54.6M | £60.6M 2024 accounts | £54.6M |
| 162 | Flavour Warehouse Limited Darwen | £60.5M 2024 accounts | — |
| 163 | Cooperscroft Care Home Limited Harrow | £60.0M | — |
| 164 | CMS Payments Intelligence Limited Manchester | £59.6M | — |
| 165 | W.H. Brakspear & Sons Limited Henley On Thames | £59.5M 2024 accounts | — |
| 166 | Qiagen Manchester Limited Manchester | £59.2M 2024 accounts | — |
| 167 | Herd Hire Ltd Addlestone Turnover £50.6M | £59.0M | £50.6M |
| 168 | Walker Modular Limited Hull | £58.7M | — |
| 169 | Aico Oswestry Turnover £173.0M | £58.7M | £173.0M |
| 170 | Newland Homes Limited Gloucester Turnover £59.0M | £57.5M 2024 accounts | £59.0M |
| 171 | DS Smith Packaging Limited London Turnover £687.2M | £57.4M 2024 accounts | £687.2M |
| 172 | Hepco Slide Systems Limited London Turnover £44.4M | £57.3M 2024 accounts | £44.4M |
| 173 | Storage on Site Limited Southampton | £57.3M | — |
| 174 | Elbit Systems UK Limited Bristol | £57.0M 2024 accounts | — |
| 175 | Galgorm Manor Hotel Limited Belfast Turnover £39.2M | £56.9M | £39.2M |
| 176 | Phoenix Me Limited London | £56.8M | — |
| 177 | One Call Insurance Services Limited Doncaster Turnover £59.3M | £56.5M 2024 accounts | £59.3M |
| 178 | Marla Tube Fittings Limited Alcester | £56.4M | — |
| 179 | Moss Bros Group Limited London Turnover £145.9M | £56.3M 2025 accounts | £145.9M |
| 180 | Plexus Corp (UK) Limited Roxburghshire | £56.0M | — |
| 181 | Day Lewis Medical Limited Croydon Turnover £280.8M | £55.3M 2024 accounts | £280.8M |
| 182 | Plasmor Limited Knottingley | £55.1M | — |
| 183 | Fairfax Plant Hire Limited Selby Turnover £22.4M | £54.8M 2023 accounts | £22.4M |
| 184 | Four Marketing Limited London Turnover £71.1M | £54.5M 2024 accounts | £71.1M |
| 185 | Country Court Care Homes Limited Spalding Turnover £18.9M | £53.9M | £18.9M |
| 186 | Willerby Limited Hull Turnover £110.3M | £53.7M | £110.3M |
| 187 | Rhenus Warehousing Solutions Ltd Nuneaton | £53.0M 2024 accounts | — |
| 188 | Secured Fixed Income PLC London | £52.8M 2025 accounts | — |
| 189 | Siderise Insulation Limited Maesteg | £52.7M 2024 accounts | — |
| 190 | Pickstock Telford Limited Telford | £52.7M 2024 accounts | — |
| 191 | G.S. Brown Construction Limited Perthshire | £52.7M 2024 accounts | — |
| 192 | Feed Factors Limited London Turnover £66.1M | £52.3M | £66.1M |
| 193 | Leekes Limited Pontyclun | £52.2M | — |
| 194 | Skyjack UK Limited Oswestry Turnover £45.4M | £52.1M 2024 accounts | £45.4M |
| 195 | Jackson Family Holdings Limited London | £52.1M 2024 accounts | — |
| 196 | Marcegaglia (UK) Limited Dudley Turnover £69.7M | £52.0M 2024 accounts | £69.7M |
| 197 | Nexgen Cloud Limited London | £51.8M 2024 accounts | — |
| 198 | M. O'Brien (Plant Hire) Ltd. Bedford | £51.7M | — |
| 199 | Eisler Capital (UK) Ltd Birmingham Turnover £64.7M | £51.5M 2019 accounts | £64.7M |
| 200 | The Kay Group (UK) Limited Blackburn | £50.9M | — |
| 201 | Renaker Build Limited Manchester Turnover £280.3M | £50.7M | £280.3M |
| 202 | Galldris Services Ltd Harrow | £50.6M | — |
| 203 | The Papworth Trust Huntingdon Turnover £14.5M | £50.5M | £14.5M |
| 204 | Spaghetti Bridge Ltd London Turnover £24.4M | £50.5M | £24.4M |
| 205 | Cameron Homes Limited Burntwood Turnover £63.5M | £50.2M 2023 accounts | £63.5M |
| 206 | Wedo Business Finance Ltd Oldham | £50.1M | — |
| 207 | Lake District Estates Company Limited Kendal Turnover £16.0M | £49.9M | £16.0M |
| 208 | Eden Supported Living Limited Newark Turnover £34.2M | £49.7M | £34.2M |
| 209 | Roper Rhodes Limited Bath | £49.5M 2021 accounts | — |
| 210 | Admiral Taverns Piccadilly Limited London Turnover £52.3M | £49.3M | £52.3M |
| 211 | Lifetime Training Group Limited Bristol Turnover £68.4M | £48.5M 2023 accounts | £68.4M |
| 212 | Brothers Drinks Co. Limited Shepton Mallet | £48.5M 2024 accounts | — |
| 213 | What More UK Limited Padiham, Burnley | £48.2M 2024 accounts | — |
| 214 | Grey Technology Limited Worcester Turnover £34.4M | £48.1M 2022 accounts | £34.4M |
| 215 | Texel Finance Limited London | £47.7M 2024 accounts | — |
| 216 | Regent Gas Limited London Turnover £63.4M | £47.6M 2021 accounts | £63.4M |
| 217 | Auto-Sleepers Group Limited Broadway Turnover £123.8M | £47.4M | £123.8M |
| 218 | Thwaites Limited Leamington Spa | £47.3M | — |
| 219 | Hippo Digital Limited Leeds Turnover £73.2M | £47.1M 2025 accounts | £73.2M |
| 220 | Kirintec Limited Ross-On-Wye | £47.0M 2023 accounts | — |
| 221 | Comfy Quilts Limited Manchester | £47.0M 2025 accounts | — |
| 222 | Evelyn Partners Investment Management Services Limited London Turnover £115.2M | £46.4M 2023 accounts | £115.2M |
| 223 | James Hargreaves(plumbers'Merchants)limited Burnley | £46.3M 2024 accounts | — |
| 224 | Collins Earthworks Limited Ripley Turnover £81.8M | £46.0M 2020 accounts | £81.8M |
| 225 | Yours Clothing Limited Peterborough | £45.9M 2025 accounts | — |
| 226 | Futamura Chemical UK Limited Wigton Turnover £55.7M | £45.9M | £55.7M |
| 227 | Mindful Chef Ltd London Turnover £55.9M | £45.7M 2024 accounts | £55.9M |
| 228 | Coastfields Leisure Limited Faversham | £45.4M 2024 accounts | — |
| 229 | Thomas Armstrong (Concrete Blocks) Limited Maryport Turnover £61.2M | £45.3M | £61.2M |
| 230 | Nextenergy Capital Limited London Turnover £17.5M | £45.3M | £17.5M |
| 231 | Cru International Limited London | £44.4M | — |
| 232 | Marex FX Limited London Turnover £29.0M | £44.4M 2023 accounts | £29.0M |
| 233 | DE Soutter Medical Limited Aylesbury | £44.3M | — |
| 234 | Aesseal PLC Mill Close Rotherham Turnover £52.5M | £44.2M 2020 accounts | £52.5M |
| 235 | Kingsmills Hotel (Inverness) Limited Aberdeen Turnover £12.3M | £44.1M 2024 accounts | £12.3M |
| 236 | Higgins Partnerships 1961 PLC Essex | £44.1M 2024 accounts | — |
| 237 | Oliver Connell and Son Limited South Greenford | £44.0M 2025 accounts | — |
| 238 | Loudwater Trade and Finance Limited London | £44.0M | — |
| 239 | Machine Mart Limited Nottinghamshire Turnover £56.1M | £43.9M | £56.1M |
| 240 | Pukka Pies Limited Leicester Turnover £58.1M | £43.8M 2021 accounts | £58.1M |
| 241 | Result Clothing Limited Colchester | £43.8M | — |
| 242 | Assa Abloy Global Solutions UK Limited Reading Turnover £61.7M | £43.7M 2024 accounts | £61.7M |
| 243 | Identity Events Management Limited East Sussex Turnover £49.2M | £43.7M 2025 accounts | £49.2M |
| 244 | RCH Care Homes Limited Ilford Turnover £28.3M | £43.7M 2022 accounts | £28.3M |
| 245 | British Bakels Limited Bicester | £43.6M 2024 accounts | — |
| 246 | Libra Textiles Limited Colne | £43.6M | — |
| 247 | Al. Murad D.I.Y. Limited Leeds Turnover £35.5M | £43.6M | £35.5M |
| 248 | North West Academies Trust Limited Chester | £43.6M | — |
| 249 | Ford & Slater Limited Leicester Turnover £364.6M | £43.6M 2024 accounts | £364.6M |
| 250 | A.C. Goatham and Son Limited Rochester | £43.4M | — |
| 251 | Steven Eagell Limited London Turnover £840.7M | £43.3M 2022 accounts | £840.7M |
| 252 | Crossroads Truck & Bus Limited Birstall, Batley | £43.0M | — |
| 253 | L.J. Fairburn and Son Limited Alford Turnover £116.4M | £42.9M | £116.4M |
| 254 | Mammoet (UK) Limited Wallsend | £42.9M | — |
| 255 | Primaflow Limited Newbury Turnover £309.3M | £42.7M 2019 accounts | £309.3M |
| 256 | A.J.N. Steelstock Ltd. Newmarket Turnover £155.8M | £42.6M | £155.8M |
| 257 | General All Purpose Plastics Limited Blackburn Turnover £157.6M | £42.5M | £157.6M |
| 258 | Mapei (UK) Limited Halesowen | £42.3M | — |
| 259 | Speed Medical Examination Services Limited Chorley | £42.2M | — |
| 260 | Crown Oil Limited Heap Bridge | £42.1M | — |
| 261 | Mba Group Limited London | £42.0M | — |
| 262 | CMS Cepcor Limited Coalville Turnover £63.3M | £41.8M | £63.3M |
| 263 | Loans 2 Go Limited Putney | £41.7M 2024 accounts | — |
| 264 | Pharmaceuticals Direct Limited Turnover £25.2M | £41.7M | £25.2M |
| 265 | Park's of Hamilton (Townhead Garage) Limited Hamilton Turnover £791.0M | £41.4M 2024 accounts | £791.0M |
| 266 | Xpate Ltd London Turnover £5.9M | £41.3M 2024 accounts | £5.9M |
| 267 | BG Lending PLC London Turnover £250.5k | £41.2M | £250.5k |
| 268 | N.D. Brown Limited Slade Heath | £41.1M 2025 accounts | — |
| 269 | Wellburn Care Homes Limited Newburn Turnover £27.5M | £41.1M | £27.5M |
| 270 | Mems Power Generation Limited Beechings Way,gillingham | £41.1M | — |
| 271 | Countrystyle Recycling Limited Sittingbourne Turnover £104.0M | £40.8M | £104.0M |
| 272 | Fletchers Solicitors Limited Manchester Turnover £34.1M | £40.8M 2022 accounts | £34.1M |
| 273 | Grocola PLC Chingford Turnover £2.1M | £40.6M | £2.1M |
| 274 | Hillhouse Quarry Group Limited Ayrshire Turnover £49.4M | £40.6M 2020 accounts | £49.4M |
| 275 | RTC Education Ltd Harrow Turnover £54.0M | £40.5M 2023 accounts | £54.0M |
| 276 | Crypto Facilities Ltd London | £40.4M | — |
| 277 | Manorview Hotels Limited Johnstone Turnover £24.2M | £40.4M 2024 accounts | £24.2M |
| 278 | Bancroft Limited London Turnover £69.6M | £40.3M 2024 accounts | £69.6M |
| 279 | Osler Diagnostics Limited Oxford | £40.3M 2020 accounts | — |
| 280 | Pavers Limited York | £40.3M 2025 accounts | — |
| 281 | Mtalx Limited Manchester | £40.2M 2024 accounts | — |
| 282 | Avondale Foods (Craigavon) Limited Craigavon | £40.1M 2024 accounts | — |
| 283 | L Jackson & Co Limited Doncaster Turnover £24.9M | £40.1M 2024 accounts | £24.9M |
| 284 | McBurney Transport Group Limited Antrim Turnover £92.0M | £40.1M 2019 accounts | £92.0M |
| 285 | Wessex Internet Limited Blandford Turnover £4.8M | £39.8M 2023 accounts | £4.8M |
| 286 | Distinct Group Ltd London Turnover £10.6M | £39.7M | £10.6M |
| 287 | Woolacombe Bay Holiday Village Limited Barnstaple Turnover £17.7M | £39.6M 2024 accounts | £17.7M |
| 288 | M J Church (Plant) Limited Chippenham | £39.4M | — |
| 289 | Adanola Limited Manchester Turnover £84.5M | £39.4M 2025 accounts | £84.5M |
| 290 | Brooks Bros. (UK) Limited Maldon | £39.4M 2024 accounts | — |
| 291 | SS&C Solutions Limited London | £39.3M 2024 accounts | — |
| 292 | Comfort Care Services (UK) Ltd Slough | £39.1M | — |
| 293 | Cuerden Estates Ltd. Wigan Turnover £30.8M | £38.9M | £30.8M |
| 294 | Routeco Limited Milton Keynes Turnover £108.6M | £38.2M | £108.6M |
| 295 | McConnel Limited Evesham | £38.2M 2024 accounts | — |
| 296 | H.Weston & Sons Limited Ledbury | £38.1M | — |
| 297 | Power Electrics (Bristol) Limited Bristol Turnover £46.4M | £38.1M 2024 accounts | £46.4M |
| 298 | Paxton Access Limited East Sussex | £38.1M | — |
| 299 | Charles Ransford & Son Limited Bishops Castle Turnover £17.5M | £37.9M | £17.5M |
| 300 | C & W Berry Limited Leyland Preston Turnover £66.7M | £37.8M | £66.7M |